RECOMBINANT BARNASE AS A LABEL IN ELISA

Recombinant barnase was proposed as a label in the enzyme-linked immun osorbent assay (ELISA). Barnase-conjugated pig transferrin was prepare d by the periodate oxidation procedure. Solid-phase bound barnase acti vity was determined from the change in RNA-ethidium bromide complex fl uorescence upon RNA hydrolysis. The sensitivity of transferrin-barnase conjugate determination in ELISA was no less than 5 ng per well. The conjugate was applied in competition ELISA for free transferrin determ ination.