F. Kunkel et G. Herrler, STRUCTURAL AND FUNCTIONAL-ANALYSIS OF THE S-PROTEINS OF 2 HUMAN CORONAVIRUS OC43 STRAINS ADAPTED TO GROWTH IN DIFFERENT CELLS, Archives of virology, 141(6), 1996, pp. 1123-1131
The receptor-binding activity of strain CU (grown in MDCK I cells) and
of strain VA (adapted to Vero cells) of human coronavirus OC43 was an
alyzed and compared with the binding activity of bovine coronavirus (B
CV) and of the OC43 strain provided by the American Type Culture Colle
ction (AT). Results obtained with resialylated erythrocytes indicated
that the ability of the viruses to recognize 9-O-acetylated sialic aci
d in an alpha 2,6-linkage decreased in the following order: AT > CU >
BCV > VA. Only minor differences were observed with respect to the alp
ha 2,3-linkage. The amino acid sequence of the S protein of strain CU
and VA was derived from the nucleotide sequence of the cloned gene. St
rain VA differed from strain CU in 34 positions, 18 in the S1 and 16 i
n the S2 subunit.