PIP2P - A TRANSCRIPTIONAL REGULATOR OF PEROXISOME PROLIFERATION IN THE YEAST SACCHAROMYCES-CEREVISIAE

Citation
H. Rottensteiner et al., PIP2P - A TRANSCRIPTIONAL REGULATOR OF PEROXISOME PROLIFERATION IN THE YEAST SACCHAROMYCES-CEREVISIAE, EMBO journal, 15(12), 1996, pp. 2924-2934
Citations number
57
Categorie Soggetti
Biology,"Cell Biology
Journal title
ISSN journal
02614189
Volume
15
Issue
12
Year of publication
1996
Pages
2924 - 2934
Database
ISI
SICI code
0261-4189(1996)15:12<2924:P-ATRO>2.0.ZU;2-M
Abstract
In Saccharomyces cerevisiae, peroxisomes are the exclusive site for th e degradation of fatty acids, Upon growth with the fatty acid oleic ac id as sole carbon source, not only are the enzymes of beta-oxidation a nd catalase A induced, but also the peroxisomal compartment as a whole increases in volume and the number of organelles per cell rises, We p reviously identified a cis-acting DNA sequence [oleate response elemen t (ORE)] involved in induction of genes encoding peroxisomal proteins, The aim of our investigation was to test whether a single mechanism a cting via the ORE coordinates the events necessary for the proliferati on of an entire organelle, Here we report the cloning and characteriza tion of the oleate-specific transcriptional activator protein Pip2p (p ip: peroxisome induction pathway), Pip2p contains a typical Zn(2)-Cys( 6) cluster domain and binds to OREs, A pip2 deletion strain is impaire d in growth on oleate as sole carbon source and the induction of beta- oxidation enzymes is abolished, Moreover, only a few, small peroxisome s per cell can be detected. These results indicate that fatty acids ac tivate Pip2p, which in turn activates the transcription of genes encod ing beta-oxidation components and acts as the crucial activator of per oxisomes.