SEPARATION OF PLANT PHENOLIC-COMPOUNDS BY CAPILLARY ZONE ELECTROPHORESIS

A capillary zone electrophoretic method with on-line UV detection was used for resolving fourteen phenolic species within 13 min by using a 100 cm x 75 mu m I.D. fused-silica tube with 0.01 M sodium berate buff er (pH 9.0) as electrolyte at 18 kV. The electrophoretic behaviour of phenolic species was investigated in order to optimize their separatio n as a function of the running buffer pH, concentration, solute inject ion method and time and applied voltage. Selectivity is strongly affec ted by the electrolyte concentration and pH. Quantitative aspects of c apillary zone electrophoresis are also discussed.