MONITORING OF THE PURIFICATION OF SYSTEMIN BY CAPILLARY ELECTROPHORESIS

Capillary electrophoresis (CE) was applied as a method for monitoring of the separation process of systemin, an endogenous peptide from toma to plants. Systemin, an 18-amino-acid polypeptide, was isolated from t omato leaves and purified. A systemin peak was identified using synthe tic systemin as a marker, and traced by CE and reversed-phase high-per formance liquid chromatography (RP-HPLC) during all chromatographic st eps. The best results of CE analysis were obtained in 25 mM phosphate buffer at acidic pH using a constant operating voltage of 30 kV.