DNA AMPLIFICATION ON CHROMOSOME 3Q26.1-Q26.3 IN SQUAMOUS-CELL CARCINOMA OF THE LUNG DETECTED BY REVERSE CHROMOSOME PAINTING

Multiple genetic lesions have been reported in small. cell lung carcin oma (SCLC), while considerably less information is available on squamo us cell. carcinoma (SCC). We used reverse chromosome painting to scree n a total of nine SCCs for DNA amplifications. In three of the nine SC Cs, hybridisation signals were found at chromosome region 3q26.1-q26.3 , which does not contain any known oncogene. In one of the three SCCs, there were additional hybridisation signals at 1q, 5p and 6p21.1. The high frequency of a consistent amplification (3q26.1-q26.3) in SCC st rongly indicates a novel gene at 3q26.1-q26.3 that is important in the pathology of SCC. Copyright (C) 1996 Elsevier Science Ltd.