ANTIPLATELET AND CALCIUM INHIBITORY PROPERTIES OF EUGENOL AND SODIUM EUGENOL ACETATE

1. Eugenol (3-methoxy-4-hydroxy-propenylbenzene) or sodium eugenol ace tate (4-O-acetic acid sodium 3-methoxy-1-propenylbenzene) (0.25, 0.5, 1 mM) concentration dependently inhibited arachidonic acid (AA), colla gen, epinephrine and ADP induced platelet aggregation. 2. Eugenol or s odium eugenol acetate inhibited collagen induced aggregation of washed rabbit plate lets synergistically with creatine phosphate/creatine ph osphokinase (CP/CPK, 5 mM/10 U/ml) or p-bromophenacyl bromide (p-BPB, 10 mu M), and they also potentiated the inhibitory action of imidazole (0.5 mM) on AA-induced aggregation. 3. Eugenol or sodium eugenol acet ate (0.25, 0.5, 1 mM) concentration-dependently inhibited AA induced t hromboxane B-2 and prostaglandin E(2) formation. 4. The rise of intrac ellular Ca2+ caused by collagen, epinephrine, ADP, and AA were inhibit ed by eugenol or sodium eugenol acetate (1 mM).