IMPROVED KINETIC RESOLUTION BY ENZYME-CATALYZED PARALLEL REACTIONS

Authors
RAKELS JLL SCHNEIDERS AGM STRAATHOF AJJ HEIJNEN JJ
Citation
Jll. Rakels et al., IMPROVED KINETIC RESOLUTION BY ENZYME-CATALYZED PARALLEL REACTIONS, Biocatalysis and biotransformation, 13(3), 1996, pp. 179-188
Citations number
17
Categorie Soggetti
Biology,"Biothechnology & Applied Migrobiology
Journal title
Biocatalysis and biotransformation → ACNP
ISSN journal
10242422
Volume
13
Issue
3
Year of publication
1996
Pages
179 - 188
Database
ISI
SICI code
1024-2422(1996)13:3<179:IKRBEP>2.0.ZU;2-6
Abstract
Competitive parallel reactions with opposite enantioselectivity are pr esented as a strategy to enhance the enantiomeric product purity in en zymatic kinetic resolution. Lipase-catalyzed simultaneous hydrolysis a nd amidation of racemic methy 12-chloropropionate led to significantly improved amide yield and enantiomeric excess. Process results can be controlled by changing the hydrolysis/amidation reaction rates through variation of the solvent and the initial amine concentration. This is described by a kinetic model.