THE ORDERED ASSEMBLY OF THE PHI-X174-TYPE PRIMOSOME .3. PRIB FACILITATES COMPLEX-FORMATION BETWEEN PRIA AND DNAT

Citation
J. Liu et al., THE ORDERED ASSEMBLY OF THE PHI-X174-TYPE PRIMOSOME .3. PRIB FACILITATES COMPLEX-FORMATION BETWEEN PRIA AND DNAT, The Journal of biological chemistry, 271(26), 1996, pp. 15656-15661
Citations number
22
Categorie Soggetti
Biology
ISSN journal
00219258
Volume
271
Issue
26
Year of publication
1996
Pages
15656 - 15661
Database
ISI
SICI code
0021-9258(1996)271:26<15656:TOAOTP>2.0.ZU;2-0
Abstract
The properties of two mutant PriA proteins, PriA C439Y and PriA. C445Y have been used to reveal the role of PriB during assembly of the phi X174-type primosome, The replication defects of both mutant PriA prote ins could be rescued by high concentrations of DnaT. Analysis of the f ormation of intermediate complexes in primosome assembly and the effec t of PriB on PriA binding to DNA demonstrated that the mutant PriA pro teins could not form a PriA-PriB complex on DNA carrying a primosome a ssembly site, Consequently, the mutant proteins also could not form Pr iA-PriB-DnaT complexes at concentrations of DnaT sufficient to form su ch a complex with wild-type PriA. In addition, PriB was found to stabi lize wild-type but not mutant PriA proteins on DNA. At high concentrat ions of DnaT, both mutant and wild-type PriA proteins could form a Pri cA-DnaT complex and support PriB-independent phi X174 complementary st rand DNA replication, Thus, during primosome assembly, PriB facilitate s complex formation between PriA and DnaT.