OCTAMER BINDING-FACTORS AND THEIR COACTIVATOR CAN ACTIVATE THE MURINEPU.1 (SPI-1) PROMOTER

PU.1 (spi-1), a member of the Ets transcription factor family, is pred ominantly expressed in myeloid and B cells, activates many B cell and myeloid genes, and is critical for development of both of these lineag es. Our previous studies (Chen, H. M., Ray-Gallet, D., Zhang, P., Heth erington, C. J., Gonzalez, D. A., Zhang, D.-E., Moreau-Gachelin, F., a nd Tenen, D. G. (1995) Oncogene 11, 1549-1560) demonstrate that the PU .1 promoter directs cell type-specific reporter gene expression in mye loid cell lines, and that PU.1 activates its own promoter in an autore gulatory loop. Here we show that the murine PU.1 promoter is also spec ifically and highly functional in B cell lines as well. Oct-1 and Oct- 2 can bind specifically to a site at base pair -55 in vitro, and this site is specifically protected in B cells in vivo. We also demonstrate that two other sites contribute to promoter activity in B cells; an S pl binding site adjacent to the octamer site, and the PU.1 autoregulat ory site. Finally, we show that the B cell coactivator OBF-1/Bob1/OCA- B is only expressed in B cells and not in myeloid cells, and that OBF- 1/Bob1/OCA-B can transactivate the PU.1 promoter in HeLa and myeloid c ells. This B cell restricted coactivator may be responsible for the B cell specific expression of PU.1 mediated by the octamer site.