DISTRIBUTION OF PHOSPHOGLYCERATE MUTASE ISOZYMES IN RAT, RABBIT AND HUMAN TISSUES

The distribution of phosphoglycerate mutase isozymes (types MM, MB and BE) in rat, rabbit and human tissues has been studied by electrophore sis on cellulose acetate and by highly-resolutive ion exchange chromat ography. In the three species, muscle is the tissue with higher phosph oglycerate mutase activity. Heart is the only tissue with the three ph osphoglycerate mutase isozymes in substantial amounts. Skeletal muscle contains mostly type MM isozyme and the other tissues possess almost exclusively type BE isozyme. Even in the presence of inhibitors, adeny late kinase can interfere with the staining reactions when large sampl es are analyzed and a long period of incubation is required.