CONSTRUCTION OF CDNA LIBRARY FROM LIVER RNA OF HEAT-SHOCKED RATS AND DNA-SEQUENCE ANALYSIS OF THE CLONE CONTAINING THE 3'-END UNTRANSLATED REGION (3'UTR) OF THE HEAT INDUCIBLE GENE HSP70.2

cDNA library constructed from liver RNA of rats subjected to hyperther mia was used to isolate divergent 3' end untranslated regions (3'UTR) of heat inducible hsp70.1 and hsp70.2 genes. As a result of a double s election procedure with the use of DNA-DNA hybridization and FCR analy sis 9 clones containing cDNA sequences derived from the 3'UTR of the h sp70.1 gene and 16 clones containing cDNA sequences derived from the 3 'UTR of the hsp70.2 gene were selected. Nucleotide sequence of the clo ned inserts was established and the Northern blot analysis was perform ed to identify the heat inducible transcript encoded by the hsp70.2 ge ne.