INDUCTION OF MICROSOMAL-ENZYMES IN LIVER OF RATS TREATED WITH CYCLOHEXANOL

The S9 fraction obtained from rats orally pretreated for 3 days with c yclohexanol was able to activate the pro-mutagen N-nitrosodimethylamin e (NDMA) into highly mutagenic metabolite(s) detected in the TA100 str ain of Salmonella typhimurium. NDMA was not mutagenic when uninduced S 9 was used as metabolic source but was approximately twice more mutage nic with cyclohexanol-induced S9 compared to ethanol-induced S9. Separ ation of microsomal proteins by sodium dodecylsulfate gel electrophore sis, displayed protein bands situated in the range of 50 000 to 52 000 molecular weight induced by both, ethanol and cyclohexanol. These res ults are evidence of the induction properties of cyclohexanol.