EVALUATION AND COMPARISON OF THE GROWTH AND VIABILITY OF YEAST-LIKE CELLS OF PARACOCCIDIOIDES-BRASILIENSIS

The growth and viability of two Paracoccidioides brasiliensis strains were evaluated in PYG and MMcM media containing no boric acid. The two P. brasiliensis strains exhibited a similar growth pattern in PYG med ium, which was considered to be good compared with the growth curves i n defined MMcM medium without boric acid. Calculation of cell generati on time in the media studied demonstrated that the cells bad greater d ifficulty in forming new generations in MMcM medium containing no bori c acid. The present data confirm the literature view that PYG medium i s the most complete in terms of nutritive substances for the growth an d development of P. brasiliensis.