CAPILLARY ELECTROPHORESIS-LASER-INDUCED FLUORESCENCE DETECTION OF AMPHETAMINE IN THE BRAIN

Prefrontal cortex microdialysis was done in rats that had received int raperitoneal amphetamine (AMPH). Samples were derivatized with 10(-4) M fluorescein isothiocyanate and incubated for 18 h. AMPH was separate d by capillary electrophoresis (CE) and detected by laser-induced fluo rescence detection (LIFD) from 30 to 150 min after injection. The limi t of mass detection was 3 amol, which is three orders of magnitude low er than that in gas chromatography-mass spectrometry, and the limit of concentration detection was 3 . 10(-9) M. The results showed that CE- LIFD is a good method for detecting AMPH in brain dialysates of rats.