Mj. Vanderschans et al., QUALITY-CONTROL OF HISTAMINE AND METHACHOLINE IN DIAGNOSTIC SOLUTIONSWITH CAPILLARY ELECTROPHORESIS, Journal of chromatography, 735(1-2), 1996, pp. 387-393
Citations number
8
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Solutions of histamine and methacholine bromide in different matrices
for diagnostic purposes were analyzed for stability and quality contro
l using capillary electrophoresis. Histamine (2-[4-imidazolyl]ethylami
ne) [CAS No. 51-45-6] was determined using a 0.1 M Tris-borate buffer
of pH 8.3 with 5 . 10(-5) M cetyltrimethylammonium bromide (CTAB) and
0.005% poly(vinyl alcohol) (PVA) and detected at 214 nm using clenbute
rol -alpha-(tert.-butylaminomethyl)-3,5-dichlorobenzyl alcohol] [37148
-27-9] as an internal standard. Metacholine bromide (acetyl-beta-metha
choline bromide) [333-31-3] was determined with a 0.01 M creatinine-ch
loride buffer of pH 4.85 and detected with indirect UV at 230 nm using
potassium as an internal standard. Histamine solutions were stable fo
r a prolonged period of time, whereas under enforced degradation condi
tions methacholine was hydrolyzed, yielding acetic acid and (tentative
ly) beta-methylcholine as reaction products.