QUALITY-CONTROL OF HISTAMINE AND METHACHOLINE IN DIAGNOSTIC SOLUTIONSWITH CAPILLARY ELECTROPHORESIS

Citation
Mj. Vanderschans et al., QUALITY-CONTROL OF HISTAMINE AND METHACHOLINE IN DIAGNOSTIC SOLUTIONSWITH CAPILLARY ELECTROPHORESIS, Journal of chromatography, 735(1-2), 1996, pp. 387-393
Citations number
8
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Volume
735
Issue
1-2
Year of publication
1996
Pages
387 - 393
Database
ISI
SICI code
Abstract
Solutions of histamine and methacholine bromide in different matrices for diagnostic purposes were analyzed for stability and quality contro l using capillary electrophoresis. Histamine (2-[4-imidazolyl]ethylami ne) [CAS No. 51-45-6] was determined using a 0.1 M Tris-borate buffer of pH 8.3 with 5 . 10(-5) M cetyltrimethylammonium bromide (CTAB) and 0.005% poly(vinyl alcohol) (PVA) and detected at 214 nm using clenbute rol -alpha-(tert.-butylaminomethyl)-3,5-dichlorobenzyl alcohol] [37148 -27-9] as an internal standard. Metacholine bromide (acetyl-beta-metha choline bromide) [333-31-3] was determined with a 0.01 M creatinine-ch loride buffer of pH 4.85 and detected with indirect UV at 230 nm using potassium as an internal standard. Histamine solutions were stable fo r a prolonged period of time, whereas under enforced degradation condi tions methacholine was hydrolyzed, yielding acetic acid and (tentative ly) beta-methylcholine as reaction products.