TOXOPLASMA-GONDII - FREEZE-PRESERVATION I N CELL-CULTURE

For a better preservation and identification of Toxoplasma gondii isol ates, we propose a new method of freezing of toxoplasma grown in THP-1 cell culture. A cystogenic strain isolated from foetal blood has been grown in these cells and frozen in liquid nitrogen. After thawing, to xoplasma recover the same growth rate and morphology in vitro and the same capacity to form brain cysts into mice compared to the initial st rains. The freezing of the cell suspension provides a simple and appro priate method for preservation of Toxoplasma gondii within in << bank >> isolates.