Jf. Lapeyre et M. Faisal, OPTIMAL CULTURE CONDITIONS FOR THE PROPAGATION OF THE OYSTER PATHOGENPERKINSUS-MARINUS (APICOMPLEXA) IN PROTEIN-DEFICIENT MEDIUM, Parasite, 3(2), 1996, pp. 147-153
The protozoan, Perkinsus marinus, acclimated and proliferated in the c
ulture medium JL-ODRP-1 without bovine serum albumin. The principal cu
lture conditions for the optimal propagation of P. marinus in this pro
tein deficient culture medium, were determined. The greatest growth ra
tes of the parasite occurred at 28 degrees C, 661 mOsm/kg and pH 7.5.
Decreasing seeding densities from 16 x 10(5) to 1 x 10(5) also increas
ed growth rate. After several passages in the absence of 5% CO2 tensio
n, the growth rate of P. marinus was similar to its original value in
the presence of 5% CO2 tension. This protein deficient culture medium
is ideally suited to study parasite-derived proteins since P. marinus
extracellular proteins in culture supernatants were produced and were
easily detected by gel electrophoresis and silver staining.