A COMPLEMENTATION OF 2 GENES ORIGINATING FROM SUSCEPTIBLE CAPSICUM-ANNUUM LINES CONFERS A NEW AND COMPLETE RESISTANCE TO PEPPER VENOUS MOTTLE VIRUS

A doubled haploid (DH) line completely resistant to pepper veinal mott le virus (PVMV) was recovered from the F-1 hybrid between two Capsicum annuum lines, Perennial and Florida VR2, whereas both of these lines were susceptible to PVMV. These observations suggest that resistance t o PVMV results from a complementary action of genes originating from P erennial and Florida VR2. F-2 plants were obtained by crossing individ ual DH lines from a hybrid (Perennial x Yolo Wonder) with Florida VR2. In 14 F-2 families, the segregation ratios observed for PVMV resistan ce were consistent with the complementation of two recessive genes, ea ch one coming from one of the parents. One gene from Florida VR2 was i dentical or tightly linked to pvr2(2) (a recessive gene conferring com plete resistance to potato virus Y [PVY] pathotypes [0 and 1]). The ot her gene from Perennial was linked to a restriction fragment length po lymorphism marker localized on chromosome Pourpre of the pepper map an d was tentatively named pvr6. The pvr2(2) gene alone conferred complet e resistance to PVY(0) and (1), and in the genomic region around the P VMV-linked marker, we previously mapped genes also involved in potyvir us resistance. Complementation between factors already involved in pot yvirus resistance conferred an enlarged spectrum of resistance to a di stantly related pathogen.