CLONING, SEQUENCING AND REGULATION OF A CDNA-ENCODING A SMALL HEAT-SHOCK PROTEIN FROM SCHIZOSACCHAROMYCES-POMBE

We have isolated a Schizosaccharomyces pombe cDNA encoding a small hea t-shock protein, designated Hsp9. The deduced amino acid sequence shar es significant homology with the Saccharomyces cerevisiae Hsp12 gene p roduct. Northern blot analysis identified a 600-base transcript which is expressed at a low level in S. pombe exponentially growing cells, b ut is strongly induced by heat-shock and upon entry into the stationar y phase. An increase in the transcript level is also observed in respo nse to glucose deprivation.