ANTIBIOTICS PRODUCTION OF CELLULOSIC WASTE WITH SOLID-STATE FERMENTATION BY STREPTOMYCES

Cellulosic waste, corncob, was used as a substrate in the production o f oxytetracycline by Streptomyces rimosus TM-55 in solid state ferment ation. Oxytetracycline was detected on the fourth day, and reached its maximum on the eighth day. During cultivation, the moisture content o f substrate increased as incubation being, and pH value increased slig htly. Optimal conditions for oxytetracycline production were an initia l pH of 5.2 to 6.3, an initial moisture content of 64 to 67%, suppleme nted with 20% (w/w) rice bran or 1.5 to 2.5% (NH4)(2)SO4 as the sole n itrogen source, 1.0% CaCO3, 2% MgSO4 7H(2)O, 0.5% KH2PO4, and 0.6 to 0 .8% aspartic acid or lysine, with incubation for 8 days at 25 to 30 de grees C. Each gram of substrate produced 10 to 11 mg of oxytetracyclin e.