Th. Tsai et al., PHARMACOKINETICS OF OSTHOLE IN RAT PLASMA USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of pharmaceutical and biomedical analysis, 14(6), 1996, pp. 749-753
A simple high-performance liquid chromatographic method was developed
to study the pharmacokinetics of osthole in rat plasma. After addition
of an internal standard (paeonol), plasma was deproteinized by aceton
itrile for sample clean-up. The drugs were separated on a reversed-pha
se column and detected by UV absorption at 323 nm. Acetonitrile-water-
diethylamine (50:50:0.1, v/v/v) (pH 3.0, adjusted with orthophosphoric
acid) was used as the mobile phase. It was applied to the pharmacokin
etic study of osthole in rats after a dose of 10 mg kg(-1) by intraven
ous administration. A biphasic phenomenon with a rapid distribution fo
llowed by a slower elimination phase was observed from the plasma conc
entration-time curve.