PLATELET-MEDIATED LYMPHOCYTE DELIVERY TO HIGH ENDOTHELIAL VENULES

Circulating lymphocytes gain access to lymph nodes owing to their abil ity to initiate rolling along specialized high endothelial venules (HE Vs). One mechanism of rolling involves L-selectin binding to periphera l node addressin (PNAd) on HEVs. Activated platelets are shown to bind to circulating lymphocytes and to mediate rolling in HEVs, in vivo, t hrough another molecule, P-selectin, which also interacts with PNAd. I n vitro, activated platelets enhanced tethering of lymphocytes to PNAd and sustained lymphocyte rolling, even in the absence of functional L -selectin. Thus, a platelet pathway operating through P-selectin provi des a second mechanism for lymphocyte delivery to HEVs.