CA2+ CALMODULIN-DEPENDENT PROTEIN-KINASE-II IN SEPTALLY KINDLED RAT BRAINS - CHANGES IN PROTEIN LEVEL, ACTIVITY AND SUBCELLULAR-DISTRIBUTION IN HIPPOCAMPUS AND CEREBRAL-CORTEX/

The protein level and the activity of Ca2+/calmodulin-dependent protei n kinase II (CaM kinase II) in homogenate from septally kindled rat br ains were quantitatively compared with those from paired controls 2 we eks after the final stimulation. The major a subunit level was decreas ed, while the activity was increased in crude homogenate from hippocam pus and parietal cortex of kindled animals, indicating an apparent inc rease in the specific activity of CaM kinase II in these regions of th e kindled brains. No such changes were observed in cerebellum. After t he separation of crude homogenate into the soluble and particulate fra ctions, the ratio of CaM kinase II activity recovered in the soluble f raction was increased in hippocampus and parietal cortex, indicating a change in subcellular distribution of CaM kinase II in the kindled st ate.