SECRETION OF PECTATE LYASES BY CELLS OF ERWINIA-CHRYSANTHEMI AND ERWINIA-CAROTOVORA VAR ATROSEPTICA

The secretion of pectate lyases was studied in the bacteria Erwinia ca rotovora var. atroseptica and Erwinia chrysanthemi grown on nutrient m edia with and without the addition of sodium polypectate as an inducer . The use of a plasmid carrying cloned genes of pectate lyases from E. chrysanthemi and providing for a high level of constitutive synthesis of these enzymes allowed the determination of the secretion rate of p ectate lyases by cells grown without the inducer. The secretion rate o f pectate lyases by Erwinia cells was 6-10 times higher in the presenc e of the inducer. Under identical conditions, the secretion of pectate lyases by E. chrysanthemi EC16 and ENA49 was ten times faster than by E. carotovora var. atroseptica 36A.