GENOMIC STRUCTURE AND CHROMOSOMAL LOCALIZATION OF THE MOUSE CDEI-BINDING PROTEIN CDEBP (APLP2) GENE AND PROMOTER SEQUENCES

The genomic structure of the mouse gene encoding the CDEBP protein has been established. The protein was initially identified on the basis o f its ability to bind the CDEI motif (GTCACATG), The same locus has be en independently described under the name APLP2, on the basis of seque nce similarities with the Amyloid Precursor Protein (APP). The exon-in tron distribution of Cdebp appears strikingly similar to that of the A pp gene in the regions encoding the conserved domains, with a divergen t structure in the other parts. The transcription start site has been localized, and sequences with promoter activity have been identified i mmediately upstream of it by their ability to direct the expression of a reporter luciferase gene in transfected cells. This region is devoi d of either TATA or CAAT boxes. The gene has been mapped to mouse chro mosome 9 by in, situ hybridization on metaphase chromosomes. (C) 1995 Academic Press,Inc.