COMBINED EXTRACTION TECHNIQUES OF TUMOR-CELLS AND LIPID PHOSPHOLIPID ASSIGNMENT BY 2-DIMENSIONAL NMR-SPECTROSCOPY/

As the cell membranes are one of the targets of drug treatment in canc er cells, their membrane composition and variations in this compositio n need to be analyzed. Gradient selected 2D-NMR inverse heteronuclear chemical shift correlations are have described, which offer optimum se nsitivity combined with a high reliability for unequivocal signal assi gnment in proton, carbon and phosphorus spectra, concerning neutral li pids and in particular phospholipids. Additionally, an extraction proc edure is presented to extract subsequently the water soluble (PCA extr act) and lipophilic metabolites (chloroform/methanol extract) from the same cell batch.