W. Negendank et al., PHOSPHOLIPID METABOLITES IN H-1-DECOUPLED P-31 MRS IN-VIVO IN HUMAN CANCER - IMPLICATIONS FOR EXPERIMENTAL-MODELS AND CLINICAL-STUDIES, Anticancer research, 16(3B), 1996, pp. 1539-1544
The use of P-31 MRS in clinical cancer research has been hampered by b
oth poor anatomic localization of spectra and poor resolution of overl
apping signals. We found that accurate localization using 3D chemical
shift imaging and improved resolution using H-1-decoupling and nuclear
Overhauser-enhancement (NOE) increased signal-to-noise and permitted
resolution of separate components within phosphomonoester (PME) and ph
osphodiester (PDE) regions. Fifty-three cancers of different types (ly
mphoma, sarcoma, adenocarcinoma) had the following common features: (1
) phosphoethanolamine the dominant PME; (2) glycerophospho-ethanolamin
e and -choline rarely detected; (3) a broad PDE signal probably from m
embrane phospholipids; and (4) prominent nucleoside triphosphates. H-1
-decoupling with NOE-enchancement permitted us to obtain new informati
on about in vivo metabolism in human cancers; generate new hypotheses
and help guide development of experimental models appropriate to test
them; and provider a firm basis with which to examine clinical uses of
P-31 MRS.