APPLICATION OF REPRODUCTIVE TECHNOLOGY TO BUFFALOS

This paper focuses on recent advances in the application of embryo tra nsfer technologies to buffalo. The early recovery rate of 0.15 transfe rable embryos has now increased to 2.0, with altered superovulation pr otocols. Lower follicular population and poor follicular development, with adverse seasonal influences explain the lower ovulatory responses . Unovulated follicles at superovulation contribute high quantities of oestrogen, altering the uterine milieu. The birth of calves using in vitro fertilisation technology and frozen-thawed embryos and determina tion of embryonic sex using a Y-specific DNA probe, are recent milesto nes achieved using these technologies.