THE ADENOMATOUS POLYPOSIS-COLI TUMOR-SUPPRESSOR PROTEIN LOCALIZES TO PLASMA-MEMBRANE SITES INVOLVED IN ACTIVE CELL-MIGRATION

Citation
Is. Nathke et al., THE ADENOMATOUS POLYPOSIS-COLI TUMOR-SUPPRESSOR PROTEIN LOCALIZES TO PLASMA-MEMBRANE SITES INVOLVED IN ACTIVE CELL-MIGRATION, The Journal of cell biology, 134(1), 1996, pp. 165-179
Citations number
39
Categorie Soggetti
Cell Biology
Journal title
ISSN journal
00219525
Volume
134
Issue
1
Year of publication
1996
Pages
165 - 179
Database
ISI
SICI code
0021-9525(1996)134:1<165:TAPTPL>2.0.ZU;2-9
Abstract
Mutations in the adenomatous polyposis coli (APC) gene are linked to p olyp formation in familial and sporadic colon cancer, but the function s of the protein are not known. We show that APC protein localizes mai nly to clusters of puncta near the ends of microtubules that extend in to actively migrating regions of epithelial cell membranes, This subce llular distribution of APC protein requires microtubules, but not acti n filaments. APC protein-containing membranes are actively involved in cell migration in response to wounding epithelial monolayers, additio n of the motorgen hepatocyte growth factor, and during the formation o f cell-cell contacts, In the intestine, APC protein levels increase at the crypt/villus boundary, where cell migration is crucial for entero cyte exit from the crypt and where cells accumulate during polyp forma tion that is linked to mutations in the microtubule-binding domain of APC protein. Together, these data indicate that APC protein has a role in directed cell migration.