CADMIUM DETERMINATION IN HUMAN WHOLE-BLOOD BY ELECTROTHERMAL ATOMIC-ABSORPTION SPECTROMETRY WITH MAGNESIUM-CHLORIDE AND SODIUM-HYDROXIDE ASA COMBINED CHEMICAL MODIFIER

In this work, we propose a method to determine trace amounts of Cd in human whole blood samples by electrothermal atomic absorption spectrom etry (ETAAS) with the combined chemical modifier including magnesium c hloride and sodium hydroxide. Prior to the ETAAS analysis, dissolution of the blood samples is accomplished using a HNO3-HClO4/double closed -vessel microwave digestion technique followed by drying of the dissol ved blood samples by means of an infrared lamp. In using this approach , a MgCl2 chemical modifier is added to the digested samples, then the y are injected into the graphite furnace for detecting the Cd level vi a atomic absorption spectrometer. Besides we used a NaOH chemical modi fier, which removed the matrix major elements through prior ashing at 1200 degrees C for 30 s, and the Cd is subsequently volatilized at 220 0 degrees C and determined by AAS. However, the proposed method can be employed to determine the of Cd level in whole blood samples by the c alibration technique and the standard-additions method. Its validity i s confirmed with two certified reference whole blood materials (Serono rm Trace Elements Whole Blood Batch no. 205052 and Batch no. 203056). By using 10 mu L injections, a detection limit of 0.052 ng mL(-1) is a chieved.