Js. Schepers et al., TRANSMITTANCE AND REFLECTANCE MEASUREMENTS OF CORN LEAVES FROM PLANTSWITH DIFFERENT NITROGEN AND WATER-SUPPLY, Journal of plant physiology, 148(5), 1996, pp. 523-529
Nitrogen is essential for crop production, but also contributes to eut
rophication of surface water and degradation of drinking water quality
. Modern corn production requires relatively large quantities of N, wh
ich are generally supplied by fertilizers. Over-application of N ferti
lizers and animal wastes frequently results in nitrate leaching. Synch
ronizing N availability with crop N need offers the potential to prote
ct the environment without sacrificing production. Tools are needed to
rapidly and easily monitor crop N status to make timely decisions reg
arding fertilizer application. Analytical and optical techniques were
evaluated with greenhouse grown corn at silking to evaluate several me
thods to monitor crop N status. A portable chlorophyll meter was used
to measure chlorophyll content of leaves by means of transmittance mea
surements. Leaf N concentration and chlorophyll meter readings were po
sitively correlated, but were also affected by water stress and hybrid
differences. Water stress decreased chlorophyll meter readings but in
creased leaf N content and diffusive resistance. Nitrogen stress decre
ased leaf N concentration, chlorophyll meter readings, and diffusive r
esistance. Both water and N stresses affected crop reflectance measure
ments. Reflectance values in the green and near IR portions of the spe
ctrum were inversely related to crop N status. Water stress increased
reflectance in red, green, and near IR wavelengths. Water stress by N
status interactions were significant for chlorophyll meter readings as
well as reflectance measurements. Both leaf reflectance and chlorophy
ll meter measurements provided a good indication of N status for adequ
ately watered plants, but the relationships were poor for plants grown
under prolonged water stress.