N. Arai et al., AN ATTEMPT TO ANALYZE FISH OTOLITHS BY IN-AIR PIXE, Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms, 109, 1996, pp. 341-344
Fish otoliths are generally composed of aragonite crystals. A large nu
mber of trace elements such as Sr, Fe, Mn, and Zn are included in the
crystal. Especially, Sr enters the crystals much among those metals in
the process of precipitation of the calcium carbonate. In addition, i
t is expected that Sr/Ca concentration ratios can indicate ambient tem
perature. In-air PIXE was adopted to analyze trace elements in otolith
s of juvenile red sea bream. Preliminary results indicated that the Sr
/Ca ratios of the otoliths increased proportional to rearing temperatu
re and that PIXE was a fast and convenient technique for determination
of several elements in fish otoliths.