MUTANTS OF PHYCOMYCES-BLAKESLEEANUS DEFECTIVE IN ACETYL-COA SYNTHETASE

Nine mutants of the filamentous fungus Phycomyces blakesleeanus have b een isolated on the basis of their resistance to fluoroacetate, None o f the isolates uses acetate as the sole carbon source, Genetic complem entation experiments revealed that all the mutants belong to the same complementation group, Biochemical analysis indicated that the acetate -induced acetyl-CoA synthetase activity is abolished in all nine mutan ts, thus suggesting that they are affected in the gene coding for acet yl-CoA synthetase (facA). (C) 1996 Academic Press, Inc.