DISTORTED SEGREGATION OF RFLP MARKERS IN REGENERATED PLANTS DERIVED FROM ANTHER CULTURE OF AN F1-HYBRID OF RICE

To examine whether parental alleles were randomly transmitted from het erozygous donor plants to anther culture (AC)-derived plants, two AC-d erived populations and one F-2 population in rice were compared for 50 RFLP markers distributed on the rice chromosomes. Two populations whi ch were developed through different anther culture methods (the ordina ry method and the direct regeneration method) and the F-2 population w ere produced from an F-1 hybrid between distantly related cultivars of Nipponbare (japonica type) and Milyang 23 (indica type). RFLP analysi s revealed that ten and eleven of the 50 markers in the two AC-derived populations showed distorted segregation ratios from the theoretical ratio of 1:1. Parental alleles were not randomly transmitted from the F-1 plant to the AC-derived plants. Additionally, the segregation rati os of seven and six RFLP markers, respectively, were distorted both fr om the 1:1 ratios and from the observed ratios in the F-2 population. The chromosomal regions involving these markers were on chromosomes 1, 3, 7, 10, 11 and 12. Some of the regions were different in the two AC -derived populations. Non-random assortment of parental alleles might be influenced by anther culture methods.