ANALYSIS OF THE ROLE OF V-REL IN TRANSCRIPTIONAL REGULATION OF HIGH-MOBILITY GROUP PROTEIN-14

The oncogene v-rel is a member of the rel/NF-kappa B family of transcr iption factors. It causes a rapidly fatal lymphoma in young chicks and is capable of transforming both fibroblasts and primitive hematopoiet ic cells in culture. To understand the role of v-rel in transformation we constructed an inducible form of v-rel and used it to identify pot ential cellular target genes for v-rel regulation. In this paper we sh ow that High Mobility Group Protein 14 (HMG 14) is expressed in a wide variety of v-rel transformed cell types. In addition we show that v-r el participates in the transcriptional regulation of HMG 14 and that e xtracts from v-relER cells interact with the HMG 14 promoter. These ex periments suggest a role for v-rel in the regulation of a unique gene whose protein product may influence gene transcription in a global fas hion.