THE COMPLETE NUCLEOTIDE-SEQUENCE OF BACTERIOPHAGE-HP1 DNA

The complete nucleotide sequence of the temperate phage HP1 of Haemoph ilus influenzae was determined, The phage contains a linear, double-st randed genome of 32 355 nt with cohesive termini, Statistical methods were used to identify 41 probable protein coding segments organized in to five plausible transcriptional units, Regions encoding proteins inv olved in recombination, replication, transcriptional control, host cel l lysis and phage production were identified, The sizes of proteins in the mature HP1 particle were determined to assist in identifying gene s for structural proteins, Similarities between HP1 coding sequences a nd those in databases, as well as similar gene organizations and contr ol mechanisms, suggest that HPI is a member of the P2-like phage famil y, with strong similarities to coliphages P2 and 186 and same similari ty to the retronphage Ec67.