COMPARISON OF PLASMID DNA PREPARATION METHODS FOR DIRECT GENE-TRANSFER AND GENETIC IMMUNIZATION

Authors
DAVIS HL SCHLEEF M MORITZ P MANCINI M SCHORR J WHALEN RG
Citation
Hl. Davis et al., COMPARISON OF PLASMID DNA PREPARATION METHODS FOR DIRECT GENE-TRANSFER AND GENETIC IMMUNIZATION, BioTechniques, 21(1), 1996, pp. 92
Citations number
28
Categorie Soggetti
Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
07366205
Volume
21
Issue
1
Year of publication
1996
Database
ISI
SICI code
0736-6205(1996)21:1<92:COPDPM>2.0.ZU;2-Q
Abstract
Plasmid DNA is widely used for direct gene transfer in animals to stud y gene therapy, gene regulation, drug delivery and genetic immunizatio n. Here we compare cesium chloride and anion-exchange purified plasmid DNA for direct gene transfer in mouse muscle and show no differences in efficiency of transfection with reporter genes or in humoral respon se to DNA-based immunization.