ANALYSIS OF THE HMW GLUTENIN SUBUNITS IN IMMATURE SEEDS FOR SELECTIONOF BREAD-MAKING LINES IN WHEAT (TRITICUM-AESTIVUM L)

The compositions of the high-molecular-weight (HMW) glutenin subunits affecting bread-making quality were analyzed in immature grains of Rec ital, Pale Duro, Flamura, Pliska, Monopol, and Nanbukomugi using sodiu m dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), While varietal differences were found in the relationship between the matur ity of grains and detection of the HMW glutenin subunits, we could det ermine all of the subunits found in mature grains using immature grain s of either of following conditions: (1) on and after 20 days after fl owering, and (2) below 55 % of water content in whole grains, In this stage, enough plants were obtained from half grains containing embryos , The results suggest that we can efficiently select breeding material s before maturity by the composition of the HMW glutenin subunits, The selection of breeding materials before maturity is useful for breedin g bread-making wheat in the Tohoku district that has a short period fr om harvest to sowing.