DEVELOPMENT OF WAXY COMMON WHEAT BY HAPLOID BREEDING

Waxy wheat cultivar with no amylose in endosperm starch has never been reported, We tried to develop waxy wheat cultivar based on a combinat ion of partial waxy mutants lacking one and two of the three proteins by haploid method of breeding in order to raise fixed lines in a short period, We used two common wheats, 'Kanto 107' lacking both the Wx-A1 and Wx-B1 proteins, and 'Bai Hue', lacking the Wx-D1 protein, Out of 19 doubled haploid (DH) plants, 2 plants were identified as waxy genot ype by iodine solution reaction and amylose content. Electrophoretic a nalysis did not detect the Wx protein in these waxy phenotypes, The ch i(2) test among seeds (glassy + floury seeds) collected at random for inheritance of the waxy character at F-2 generation, indicated that th e observed frequency was not significantly different from the expected 63:1 segregation ratio, In 1000 floury F-2 seeds, however, the chi(2) test indicated that this frequency was significantly different from t he expected 63:1 segregation ratio, Namely, waxy seeds were observed m ore than the expected segregation ratio in floury F-2 seeds, One (C-D 1478) of two waxy wheats was similar to the male parent,'Bai Hue' exce pt the culm length, while the other (C-D 1479) was similar to the fema le parent, 'Kanto 107'.