DIFFERENT PRLA PROTEINS INCREASE THE EFFICIENCY OF PERIPLASMIC PRODUCTION OF HUMAN INTERLEUKIN-6 IN ESCHERICHIA-COLI

Authors
PEREZPEREZ J BARBERO JL MARQUEZ G GUTIERREZ J
Citation
J. Perezperez et al., DIFFERENT PRLA PROTEINS INCREASE THE EFFICIENCY OF PERIPLASMIC PRODUCTION OF HUMAN INTERLEUKIN-6 IN ESCHERICHIA-COLI, Journal of biotechnology, 49(1-3), 1996, pp. 245-247
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Journal of biotechnology → ACNP
ISSN journal
01681656
Volume
49
Issue
1-3
Year of publication
1996
Pages
245 - 247
Database
ISI
SICI code
0168-1656(1996)49:1-3<245:DPPITE>2.0.ZU;2-1
Abstract
The export efficiency of a fusion of the Escherichia coli preOmpA sign al peptide to human interleukin-6 can be significantly raised by coexp ressing three different prlA alleles of secY along with wild type secE . The effect seems prlA-specific, as prlGl (a prl allele of secE) does not affect the export of preOmpA-hIL-6. Coexpression of secD and secF also stimulates the export of the fusion protein.