CHARACTERIZATION OF PHENOLIC AND SECOIRIDOID AGLYCONS PRESENT IN VIRGIN OLIVE OIL BY GAS-CHROMATOGRAPHY CHEMICAL-IONIZATION MASS-SPECTROMETRY

Olive oil phenolic and secoiridoid compound derivatives were detected by mass spectrometry, Chemical ionization (CI) allowed us to obtain pa rent ions, that were not detectable in the electron impact mode. CI ex periments were performed using either CH4 or NH3 as the reactant gas. Unlike CH4-CI, which fails to give molecular mass information, NH3-CI provided, in all the cases explored, an adduct ion [M+18](+) that was sufficiently stable to be detected and recorded. Information about mol ecular masses was very useful for the complete characterization of agl ycons from glycosides occurring in virgin olive oils. Aglycons of ligs troside, of decarbomethoxyoleuropein and of oleuropein were detected. Each aglycon, because of several tautomeric equilibria involving the r ing opening of secoiridoid, showed up as compounds with four main stru ctures after derivatization with bis(trimethylsilyl)trifluoracetamide.