THE EFFECTS OF ASCORBIC-ACID AND IRON CO-SUPPLEMENTATION ON THE PROLIFERATION OF 3T3 FIBROBLASTS

Citation
Cs. Collis et al., THE EFFECTS OF ASCORBIC-ACID AND IRON CO-SUPPLEMENTATION ON THE PROLIFERATION OF 3T3 FIBROBLASTS, Free radical research, 25(1), 1996, pp. 87-93
Citations number
23
Categorie Soggetti
Biology
Journal title
ISSN journal
10715762
Volume
25
Issue
1
Year of publication
1996
Pages
87 - 93
Database
ISI
SICI code
1071-5762(1996)25:1<87:TEOAAI>2.0.ZU;2-B
Abstract
Exposure of 3T3 fibroblasts to Fe-II reveals a concentration-dependent inhibition of cell proliferation compared to control cells, the appar ent threshold for this iron-mediated effect being 5 mu M Fe-II. The in hibition of cell proliferation was accompanied by an enhancement of to tal malondialdehyde (MDA) levels (as detected directly by hplc) in the cells at higher iron concentrations. The co-supplementation of Fe-II with varying concentrations of ascorbic acid over the range 5 mu M to 240 mu M had no significant effect on the threshold for iron toxicity or lipid peroxidation. These results show that there is neither a sign ificant exacerbation of the pro-oxidant effect of Fe-II nor any protec tive effect of ascorbate when cultures of 3T3 mouse fibroblasts are ex posed to co-supplementation regimes of iron with ascorbic acid.