EFFECT OF EXCRETA, BLOOD AND VITELLUS CONTAMINATION ON FERTILIZATION SUCCESS OF ONCORHYNCHUS-MYKISS

Egg batches are often contaminated by broken eggs, blood, faeces or ur ine, This study was conducted to determine what effect these contamina nts have on fertilisation success, and also to determine the best dilu ent to use for fertilisation. Fertilisation successes were only negati vely influenced when egg batches were intentionally contaminated with broken eggs, while contamination with blood, faeces or urine showed de creases which were not critical. Ovarian fluid was the best diluent to use when egg batches were contaminated by blood, faeces or urine, but not when contaminated with broken eggs, If egg batches are contaminat ed with broken eggs, it is advisable to use a salt solution, for examp le borax. Salt diluents keep the globulin in solution, which prevents blockage of the micropyle, The other possibilities are to prevent any eggs being broken during the stripping process by using well-trained s trippers, to use a good stripping technique and to ensure that the egg s are handled carefully during the fertilisation procedure.