Background: The major cause of fetal hemolytic disease is maternal imm
unization to D in D-incompatible pregnancies. To prevent complications
, D-incompatible pregnancies are monitored for the level of maternal a
nti-D. At present, the monitoring of anti-D levels is performed by the
indirect antiglobulin test complemented by quantitation by the techni
que used in an automated antibody detection and quantitation instrumen
t. Study Design and Methods: Flow cytometry was used to quantitatively
determine the level of anti-D in serum and to analyze the IgG subclas
s distribution and the presence of IgM anti-D in these samples. The re
sults were compared to the indirect antiglobulin test titer and to the
results obtained by the technique used in an automated antibody detec
tion and quantitation instrument. Results: Flow cytometry allowed sens
itive and accurate determinations of anti-D levels with low interassay
and intra-assay variability, both for serum samples and standard curv
es. Conclusion: Flow cytometry is a simple, rapid, and reliable method
for determining the serum levels of D antibodies and their Ig subclas
s distribution. It is therefore well suited for the monitoring of wome
n during D-incompatible pregnancies.