Sprouts of cvs Arran Chief, Pentland Kappa and Golden Wonder were inoc
ulated with fresh wart tissue using the Glynne-Lemmerzahl (GL) method
and results of tests carried out using five different incubation regim
es were compared. When tubers of Arran Chief and Pentland Kappa were i
ncubated uncovered in the light, rotting of sprouts was reduced and th
ere was more extensive development of summer sporangia. Similar result
s were obtained on a range of cultivars when inoculations were done us
ing summer or winter spores. Winter spores, which germinate freely in
distilled water, can readily be extracted from dried rotted wart tissu
e to provide a reliable and continuous supply of inoculum. At the Scot
tish Agricultural Science Agency, wart susceptibility testing using th
e GL method has been done with winter spores since 1987.