CLONING AND EXPRESSION OF A NOVEL NEUROPEPTIDE-Y RECEPTOR

The neuropeptide Y family of peptides, which includes neuropeptide Y ( NPY), peptide YY (PYY), and pancreatic polypeptide (PP), are found in the central and peripheral nervous system and display a wide array of biological activities. These actions are believed to be mediated throu gh pharmacologically distinct G protein-coupled receptors, and, to dat e, three members of the NPY receptor family have been cloned. In this study we describe the cloning and expression of a novel NPY receptor f rom mouse genomic DNA. This receptor, designated NPY Y5, shares 60% am ino acid identity to the murine NPY Y1 receptor. The pharmacology of t his novel receptor resembles that of the NPY Y1 receptor and is distin ct from that described for the NPY Y2, Y3, and Y4 receptors. In situ h ybridization of mouse brain sections reveals expression of this recept or within discrete regions of the hypothalamus including the suprachia smatic nucleus, anterior hypothalamus, bed nucleus stria terminalis, a nd the ventromedial nucleus with no localization apparent elsewhere in the brain.