EFFECTS OF TRUNCATION OF THE COOH-TERMINAL REGION OF A NA-INDEPENDENTNEUTRAL AND BASIC-AMINO-ACID TRANSPORTER ON AMINO-ACID-TRANSPORT IN XENOPUS OOCYTES()

Citation
K. Miyamoto et al., EFFECTS OF TRUNCATION OF THE COOH-TERMINAL REGION OF A NA-INDEPENDENTNEUTRAL AND BASIC-AMINO-ACID TRANSPORTER ON AMINO-ACID-TRANSPORT IN XENOPUS OOCYTES(), The Journal of biological chemistry, 271(28), 1996, pp. 16758-16763
Citations number
29
Categorie Soggetti
Biology
ISSN journal
00219258
Volume
271
Issue
28
Year of publication
1996
Pages
16758 - 16763
Database
ISI
SICI code
0021-9258(1996)271:28<16758:EOTOTC>2.0.ZU;2-R
Abstract
To determine the role of a neutral and basic amino acid transporter (N EAT) in amino acid transport, we microinjected several COOH-terminal d eletion mutants of NEAT cRNA into Xenopus oocytes and measured transpo rt activity for arginine, leucine, and cystine in the presence and abs ence of sodium, Wild type NEAT significantly stimulated the uptake of all three amino acids 10-20-fold compared with controls, On the other hand, no mutant, except a Delta 511-685 mutant, stimulated the uptake of these amino acids, The Delta 511-685 mutant significantly increased the uptake of arginine. In the presence of sodium, the Delta 511-685 mutant also increased the uptake of leucine, The Delta 511-685 mutant did not stimulate cystine uptake in the presence or absence of sodium, The stimulation of arginine uptake by the Delta 511-685 mutant was in hibited by a 100-fold excess of unlabeled leucine in the presence of s odium, Inhibition of L-arginine uptake by L-homoserine was seen only i n the presence of sodium, and an increase in the inhibition of L-argin ine uptake by L-histidine was seen when the extracellular pH was decre ased, Furthermore, an inward current in oocytes injected with the Delt a 511-685 mutant was recorded electrophysiologically when basic amino acids were applied, Homoserine was also taken up, but sodium was neces sary for their transport, These properties of the Delta 511-685 mutant correspond to those of the y(+) amino acid transporter, If NEAT is a component of the b(0,+)-like amino acid transport system, it is unlike ly that a mutant protein (Delta 511-685) is able to stimulate an endog enous y(+)-like transport system, These results suggest that NEAT func tions as a activator of the amino acid transport system in Xenopus ooc ytes.