THE EFFECT OF MODERATE PROTEIN-MALNUTRITION ON MURINE T-CELL CYTOKINEPRODUCTION

Authors
Citation
Sb. Zhang et Tm. Petro, THE EFFECT OF MODERATE PROTEIN-MALNUTRITION ON MURINE T-CELL CYTOKINEPRODUCTION, Nutrition research, 17(1), 1997, pp. 51-64
Citations number
50
Categorie Soggetti
Nutrition & Dietetics
Journal title
ISSN journal
02715317
Volume
17
Issue
1
Year of publication
1997
Pages
51 - 64
Database
ISI
SICI code
0271-5317(1997)17:1<51:TEOMPO>2.0.ZU;2-S
Abstract
T cell responses and cytokine production requires recognition of antig enic peptides/MHC by the TCR/CD3 complex and reception of APC costimul atory signals through the CD28 counter-receptors. Protein malnutrition (PM) alters T cell immune responses and decreases resistance to infec tion. To determine the effect of PM on T cell cytokine production, int erferon-gamma (IFN)-gamma, interleukin (IL)-2 and IL-4 production by s plenic T cells from mice fed either a moderately protein deficient die t (4% casein) or a diet sufficient in protein (20% casein) in response to ConA or anti-CD3 was measured. The production of IL-4 was signific antly decreased while production of IL-2 was increased from T cells of PM mice during co-culture with native APC. To determine the effect of PM on costimulatory and counter-receptor signals, T cells were stimul ated with Concanavalin A (ConA) and costimulated with native APC, with APC from the reciprocal dietary group, or with antibody to CD28. APC from PM mice co-cultured with T cells of control mice retained a norma l ability to costimulate,control T cell cytokine production. However p urified T cells from PM mice produced significantly less cytokines whe n co-cultured with control APC.,In the absence of APC but in the prese nce of artificial costimulation by anti-CD28, the production of IL-2 a nd IL-4 from enriched T cells of PM mice was significantly decreased c ompared with T cells from control mice. Thus, the T cell counter-recep tor pathway is significantly impaired, while APC costimulatory functio n remains normal. during PM. Copyright (C) 1996 Elsevier Science Inc.