MULTIPLEX AMPLIFICATION AND TYPING PROCEDURE FOR THE LOCI D1S80 AND AMELOGENIN

A method has been developed that enables multiplex amplification and s imultaneous typing of the loci D1S80 and amelogenin using discontinuou s polyacrylamide gel electrophoresis and silver staining. The protocol is sensitive, simple, rapid, and relatively inexpensive. The results of the multiplex analysis of the D1S80 and amelogenin loci were compar able to those obtained when each locus was analyzed individually. A sm all validation study was undertaken to evaluate the forensic applicabi lity of this multiplex system. The data demonstrate that DNA exposed t o a variety of environmental insults yields reliable multiplex typing results.